KRAS Antibody (D113) [DyLight 405] - Mutant

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43583V

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # D113

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all KRAS Antibodies »

Product Specifications

Immunogen

The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'. (Uniprot# P01116-2)

Specificity

This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37% pancreatic cancers and over 12% of colorectal cancers.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Applications for KRAS Antibody (D113) [DyLight 405] - Mutant

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: KRAS

KRAS, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Alternate Names

cellular c-Ki-ras2 proto-oncogene, c-Ki-ras, c-K-ras, GTPase KRas, Ki-Ras, Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog, K-Ras 2, K-ras p21 protein, KRAS1, K-RAS2A, K-RAS2B, KRAS2NS3, K-RAS4A, K-RAS4B, NS, oncogene KRAS2, PR310 c-K-ras oncogene, RASK2c-Kirsten-ras protein, transforming protein p21, v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Gene Symbol

KRAS

Additional KRAS Products

Product Documents for KRAS Antibody (D113) [DyLight 405] - Mutant

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KRAS Antibody (D113) [DyLight 405] - Mutant



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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