Kremen-2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22339

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Kremen-2 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Kremen-2. The immunogen is located within amino acids 390-440 of Kremen-2. Amino Acid Sequence: ALGASRGGPRRSWAV

Specificity

Multiple isoforms of Kremen-2 are known to exist. Kremen-2 antibody is predicted to not cross-react with other Kremen protein family members. Human Kremen-2 has 6 isoforms, including isoform 1 (462aa, 49kD), isoform 2 (424aa, 45kD), isoform 3 (420aa, 44kD), isoform 4 (399aa, 42kD), isoform 5 (423aa, 45kD), and isoform 6 (381aa, 40kD). Mouse Kremen-2 has one isoform (461aa, 49kD) and rat Kremen-2 also has one isoform (468aa, 50kD). NBP2-22339 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Kremen-2 Antibody - BSA Free

Western Blot: Kremen-2 AntibodyBSA Free [NBP2-22339]

Western Blot: Kremen-2 AntibodyBSA Free [NBP2-22339]

Western Blot: Kremen-2 Antibody [NBP2-22339] - Western Blot Validation in Mouse Spleen Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: KREMEN2 NBP2-22339 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: Kremen-2 Antibody [NBP2-22339] -

Immunocytochemistry/Immunofluorescence: Kremen-2 Antibody [NBP2-22339] - KREMEN2 in HeLa cells with KREMEN2 antibody at 5 ug/ml.
Western Blot: Kremen-2 AntibodyBSA Free [NBP2-22339]

Western Blot: Kremen-2 AntibodyBSA Free [NBP2-22339]

Western Blot: Kremen-2 Antibody [NBP2-22339] - Independent Antibody Validation via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Kremen-2 [NBP2-22339] (2 ug/ml), Kremen-2 antibody from Company B (1 ug/ml) and beta-actin [NBP1-76692] (2 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Kremen-2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kremen-2

KREMEN2 encodes a high-affinity dickkopf homolog 1 (DKK1) transmembrane receptor that functionally cooperates with DKK1 to block wingless (WNT)/beta-catenin signaling. The encoded protein forms a ternary membrane complex with DKK1 and the WNT receptor lipoprotein receptor-related protein 6 (LRP6), and induces rapid endocytosis and removal of LRP6 from the plasma membrane. It contains extracellular kringle, WSC, and CUB domains. Alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. [provided by RefSeq]

Long Name

Kringle-containing Transmembrane Protein 2

Alternate Names

Kremen2

Gene Symbol

KREMEN2

UniProt

NP_757384 (Human)

Additional Kremen-2 Products

Product Documents for Kremen-2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Kremen-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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