Key Product Details

Species Reactivity

Epitope Tag

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all KT3 Epitope Tag Antibodies »

Product Specifications

Immunogen

Goats were immunized with KT3 (KPPTPPPEPET) conjugated to KLH.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for KT3 Epitope Tag Antibody - BSA Free

Western Blot: KT3 Epitope Tag AntibodyBSA Free [NB110-40654]

Western Blot: KT3 Epitope Tag AntibodyBSA Free [NB110-40654]

Western Blot: KT3 Epitope Tag Antibody [NB110-40654] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).

Applications for KT3 Epitope Tag Antibody - BSA Free

Application
Recommended Usage

ELISA

1:1000 - 1:30000

Immunocytochemistry/ Immunofluorescence

1:100-1:400

Immunoprecipitation

1 - 4 ug/mg lysate

Western Blot

1:1000 - 1:30000
Application Notes
ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: KT3 Epitope Tag

The KT3 epitope tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized KT3 epitope represents the amino acid sequence KPPTPPPEPET derived from the Simian Virus 40 (SV40) large T-antigen.

Alternate Names

KPPTPPPEPET Epitope Tag, KPPTPPPEPET Tag, KT3 Epitope Tag

Additional KT3 Epitope Tag Products

Product Documents for KT3 Epitope Tag Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for KT3 Epitope Tag Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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