Ku80/XRCC5 Antibody (5C5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37583

Novus Biologicals, part of Bio-Techne
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NBP2-37583
NBP2-37583-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5C5

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Ku80/XRCC5 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Ku80/XRCC5 expressed in E. Coli.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ku80/XRCC5 Antibody (5C5) - BSA Free

Western Blot: Ku80/XRCC5 Antibody (5C5)BSA Free [NBP2-37583]

Western Blot: Ku80/XRCC5 Antibody (5C5)BSA Free [NBP2-37583]

Western Blot: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis using XRCC5 mouse mAb against Hela (1), MCF-7 (2), A549 (3) and NIH/3T3 (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (5C5) - BSA Free [NBP2-37583]

Immunocytochemistry/ Immunofluorescence: Ku80/XRCC5 Antibody (5C5) - BSA Free [NBP2-37583]

Immunocytochemistry/Immunofluorescence: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of Hela cells using XRCC5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (5C5) - BSA Free [NBP2-37583]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (5C5) - BSA Free [NBP2-37583]

Immunohistochemistry-Paraffin: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Analysis of human tonsil tissues (left) and human colon cancer tissues (right) using XRCC5 mouse mAb with DAB staining.
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Applications for Ku80/XRCC5 Antibody (5C5) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ku80/XRCC5

Ku80, also known as XRCC5, plays a role in many diverse processes including cell signaling, proliferation, DNA repair, replication, transcriptional activation and apoptosis.

As one half of the heterodimer complex Ku, Ku80 is required for proper maintenance of the telomeric C strand. Ku80 also plays a role in DNA repair as an essential subunit of DNA-dependent protein kinase (DNA-PK) that phosphorylates certain transcription factors (Sp1, Oct-1 and p53). The heterodimer Ku binds double stranded DNA breaks for non-homologous end joining repair. Ku mutants are defective in T-DNA integration and over-expression confers increased resistance to DNA damage agents and increased susceptibility to T-DNA transformation.

Long Name

Lupus Ku Autoantigen Protein 86

Alternate Names

CTC85, G22P2, KARP1, Ku86, KUB2, NFIV, XRCC5

Gene Symbol

XRCC5

Additional Ku80/XRCC5 Products

Product Documents for Ku80/XRCC5 Antibody (5C5) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ku80/XRCC5 Antibody (5C5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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