Kynureninase Antibody (062)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89680

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 062

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Kynureninase Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Kynureninase (Uniprot#: Q16719; Met1-Asn465).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Kynureninase Antibody (062)

Western Blot: Kynureninase Antibody (062) [NBP2-89680]

Western Blot: Kynureninase Antibody (062) [NBP2-89680]

Western Blot: Kynureninase Antibody (062) [NBP2-89680] - Lane A: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa
Immunocytochemistry/ Immunofluorescence: Kynureninase Antibody (062) [NBP2-89680]

Immunocytochemistry/ Immunofluorescence: Kynureninase Antibody (062) [NBP2-89680]

Immunocytochemistry/Immunofluorescence: Kynureninase Antibody (062) [NBP2-89680] - Staining of KYNU in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KYNU monoclonal antibody (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunoprecipitation: Kynureninase Antibody (062) [NBP2-89680]

Immunoprecipitation: Kynureninase Antibody (062) [NBP2-89680]

Immunoprecipitation: Kynureninase Antibody (062) [NBP2-89680] - Lane A:0.5 mg A549 Whole Cell Lysate 0.5 uL anti-KYNU-HIS rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-KYNU-HIS rabbit monoclonal antibody,at 1:500 dilution Secondary antibody:Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size: 52 kDa

Applications for Kynureninase Antibody (062)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Kynureninase

KYNU, also known as Kynureninase, has a 465 amino acid long isoform that is 52 kDa and a short 307 amino acid that is 35 kDa; its highest expressed levels are found in placenta, liver and lung; is a PLP dependent enzyme that catalyses the cleavage of kynurenine (Kyn) into anthranilic acid (Ant), and It can also act on 3hKyn (to produce 3hAnt) and some other (3-arylcarbonyl)-alanines, and has cysteine-conjugate-beta-lyase activity. This protein is currently being studied for research on the following diseases and disorders: transient cerebral ischemia, vitamin b deficiency, hydroxykynureninuria, pellagra, cerebritis, ischemia, atopic dermatitis, intrahepatic cholangiocarcinoma, essential hypertension, noma, brain injury, dermatitis, psoriasis cholangiocarcinoma, neurologic diseases, interferon, and neuronitis. Interactions with KYNU protein have been shown to involve 40 proteins including GCK, PIN1, NEDD4L, HSPA9, and ETFA in tryptophan catabolism, metabolism, metabolism of amino acids and derivatives, and NAD metabolism pathways.

Alternate Names

KYNU, L-kynurenine hydrolase

Gene Symbol

KYNU

Additional Kynureninase Products

Product Documents for Kynureninase Antibody (062)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Kynureninase Antibody (062)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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