L1CAM Antibody (014)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89294

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA Detection

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 014

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all L1CAM Antibodies »

Product Summary for L1CAM Antibody (014)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human L1CAM (Accession#: NP_000416.1; Met1-Glu1120).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for L1CAM Antibody (014)

Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (014) [NBP2-89294]

Immunocytochemistry/ Immunofluorescence: L1CAM Antibody (014) [NBP2-89294]

Immunocytochemistry/Immunofluorescence: L1CAM Antibody (014) [NBP2-89294] - Staining of Human CD171 in Hela cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human CD171 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor (R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294]

Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294]

Immunohistochemistry-Paraffin: L1CAM Antibody (014) [NBP2-89294] - Staining of human CD171 in human appendix with rabbit monoclonal antibody (1:200). Positive staining was localized to myenteric nerve plexus.
Flow Cytometry: L1CAM Antibody (014) [NBP2-89294]

Flow Cytometry: L1CAM Antibody (014) [NBP2-89294]

Flow Cytometry: L1CAM Antibody (014) [NBP2-89294] - Analysis of human CD171 expression on HeLa cells. Cells were stained with purified anti-Human CD171, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Applications for L1CAM Antibody (014)

Application
Recommended Usage

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Sandwich ELISA Detection

1:1000-1:10000
Application Notes
This antibody will detect Human L1CAM in ELISA pair set (Catalog: # NBP2-79446). In a sandwich ELISA, it can be used as a detection antibody when paired with (Catalog: # NBP2-89292).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: L1CAM

The L1CAM gene, which is located in Xq28, is involved in three distinct conditions: 1) HSAS (hydrocephalus-stenosis of the aqueduct of Sylvius); 2) MASA (mental retardation, aphasia, shuffling gait, adductus thumbs); and 3) SPG1 (spastic paraplegia). The L1, neural cell adhesion molecule (L1CAM) also plays an important role in axon growth, fasciculation, neural migration and in mediating neuronal differentiation. Expression of L1 protein is restricted to tissues arising from neuroectoderm.

Long Name

Cell Adhesion Molecule L1

Alternate Names

CAML1, CD171, HSAS, HSAS1, MASA, MIC5, NCAM-L1, S10, SPG1

Gene Symbol

L1CAM

Additional L1CAM Products

Product Documents for L1CAM Antibody (014)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for L1CAM Antibody (014)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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