Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1A4

Format

BSA Free

Concentration

1 mg/ml

View all Lactate Dehydrogenase A/LDHA Antibodies »

Product Specifications

Immunogen

Recombinant human Lactate Dehydrogenase A/LDHA (1-332aa) purified from E.coli (NP_005557)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4)BSA Free [NBP1-74023]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4)BSA Free [NBP1-74023]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - Lane 1.: Jurkat cell lysate Lane 2.: 293T cell lysate Lane 3.: A431 cell lysate. antibody (1:1000).
Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free [NBP1-74023]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free [NBP1-74023]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody (1A4) [NBP1-74023] - HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human LDHA antibody (1:500) with Texas Red (Red).

Applications for Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:500

Western Blot

1:1000-1:8000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

Lactate through lactate dehydrogenase A (LDHA), which is encoded by a target gene of c-Myc and hypoxia-inducible factor (HIF-1). LDHA expression is repressed by SugR in the absence of sugar. Reduction of LDHA causes bioenergetic and oxidative stress leading to cell death. LDHA (LDH-5, M-LDH, or A4), which is the predominant form in skeletal muscle, kinetically favors the conversion of pyruvate to lactate. Therefore, LDH-A is an attractive target for cancer therapy since its expression is largely relegated to skeletal muscle.

Alternate Names

LDH1, LDHA, LDHM

Entrez Gene IDs

3939 (Human)

Gene Symbol

LDHA

UniProt

P00338 (Human)

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody (1A4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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