Lactate Dehydrogenase A/LDHA Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48336B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-48336AF350

Alexa Fluor 405

NBP1-48336AF405

Alexa Fluor 488

NBP1-48336AF488

Alexa Fluor 532

NBP1-48336AF532

Alexa Fluor 594

NBP1-48336AF594

Alexa Fluor 647

NBP1-48336AF647

Alexa Fluor 700

NBP1-48336AF700

Alexa Fluor 750

NBP1-48336AF750

Allophycocyanin

NBP1-48336APC

CoraFluor 1

NBP1-48336CL1

DyLight 350

NBP1-48336UV

DyLight 405

NBP1-48336V

DyLight 488

NBP1-48336G

DyLight 550

NBP1-48336R

DyLight 594

NBP1-48336DL594

DyLight 650

NBP1-48336C

DyLight 680

NBP1-48336FR

DyLight 755

NBP1-48336IR

FITC

NBP1-48336F

HRP

NBP1-48336H

Janelia Fluor 525

NBP1-48336JF525

Janelia Fluor 549

NBP1-48336JF549

Janelia Fluor 585

NBP1-48336JF585

Janelia Fluor 635

NBP1-48336JF635

Janelia Fluor 646

NBP1-48336JF646

Janelia Fluor 669

NBP1-48336JF669

mFluor Violet 450 SE

NBP1-48336MFV450

mFluor Violet 500 SE

NBP1-48336MFV500

mFluor Violet 610 SE

NBP1-48336MFV610

PE

NBP1-48336PE

PerCP

NBP1-48336PCP

Forumulation

Catalog #

BSA Free

NBP1-48336

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Porcine, Bovine

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lactate Dehydrogenase A/LDHA Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a C-terminal portion of the human Lactate Dehydrogenase A protein (within residues 280-332). [Swiss-Prot# P00338]

Reactivity Notes

Porcine reactivity reported in scientific literature (Elgin et al).

Localization

Cytoplasm.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Lactate Dehydrogenase A/LDHA Antibody [Biotin]

Application

Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lactate Dehydrogenase A/LDHA

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycoysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that cause deficiency in LDH-A has been implicated in exertional myoglobinuria (3).

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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