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Lactate Dehydrogenase A/LDHA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19320

Novus Biologicals, part of Bio-Techne
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NBP2-19320

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (91%), Chimpanzee (100%), Porcine (90%), Rabbit (91%), Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Lactate Dehydrogenase A/LDHA. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (80%).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lactate Dehydrogenase A/LDHA Antibody

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Analysis of methanol-fixed A431, using LDHA antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Immunohistochemistry-Paraffin: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - Mouse liver. LDHA stained by LDHA antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320]

Western Blot: Lactate Dehydrogenase A/LDHA Antibody [NBP2-19320] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 10% SDS-PAGE LDHA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Lactate Dehydrogenase A/LDHA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase A/LDHA

The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq]

Alternate Names

LDH1, LDHA, LDHM

Gene Symbol

LDHA

UniProt

Additional Lactate Dehydrogenase A/LDHA Products

Product Documents for Lactate Dehydrogenase A/LDHA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lactate Dehydrogenase A/LDHA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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