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Lactate Dehydrogenase B Antibody (15)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43433

Novus Biologicals, part of Bio-Techne
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NBP3-43433

Key Product Details

Species Reactivity

Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 15

Concentration

1 mg/ml

Product Specifications

Immunogen

Immunized with recombinant Rat Lactate Dehydrogenase B (Uniprot#: P42123; Met1~Leu334)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for Lactate Dehydrogenase B Antibody (15)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-20 ug/mL

Immunohistochemistry

5-20 ug/mL

Western Blot

0.5-2ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH7.4, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lactate Dehydrogenase B/LDHB

Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).

Alternate Names

Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5

Gene Symbol

LDHB

Additional Lactate Dehydrogenase B/LDHB Products

Product Documents for Lactate Dehydrogenase B Antibody (15)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lactate Dehydrogenase B Antibody (15)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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