Lactate Dehydrogenase B Antibody (6B5I2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16569
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 6B5I2
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lactate Dehydrogenase B (P07195). MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lactate Dehydrogenase B Antibody (6B5I2)
Western Blot: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569]
Western Blot: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Western blot analysis of extracts of various cell lines, using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569]
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Immunohistochemistry of paraffin-embedded human appendix using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569]
Immunohistochemistry-Paraffin: Lactate Dehydrogenase B Antibody (6B5I2) [NBP3-16569] - Immunohistochemistry of paraffin-embedded rat ovary using Lactate Dehydrogenase B Rabbit mAb (NBP3-16569) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Lactate Dehydrogenase B Antibody (6B5I2)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Lactate Dehydrogenase B/LDHB
Alternate Names
Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5
Gene Symbol
LDHB
Additional Lactate Dehydrogenase B/LDHB Products
Product Documents for Lactate Dehydrogenase B Antibody (6B5I2)
Product Specific Notices for Lactate Dehydrogenase B Antibody (6B5I2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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