Lactate Dehydrogenase B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-53421
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant human Lactate Dehydrogenase B protein (amino acids 1-93) [P07195]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lactate Dehydrogenase B Antibody - BSA Free
Knockout Validated: Lactate Dehydrogenase B Antibody - BSA Free [NBP2-53421]
Knockout Validated: Lactate Dehydrogenase B Antibody [NBP2-53421] - Western blot shows lysates of 293 human embryonic kidney parental cell line and LDHB knockout (KO) 293 cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human LDHB Polyclonal Antibody (NBP2-53421) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). Specific band was detected for LDHB at approximately 35 kDa (as indicated) in the parental 293 cell line, but is not detectable in the knockout 293 cell line. This experiment was conducted under reducing conditions.Western Blot: Lactate Dehydrogenase B AntibodyBSA Free [NBP2-53421]
Western Blot: Lactate Dehydrogenase B Antibody [NBP2-53421] - Total protein from human HeLa and A431 cells, mouse MEF cells and rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-LDHB in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.Immunocytochemistry/ Immunofluorescence: Lactate Dehydrogenase B Antibody - BSA Free [NBP2-53421]
Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase B Antibody [NBP2-53421] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-LDHB at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.Applications for Lactate Dehydrogenase B Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
2-5 ug/ml
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry
1:400
Immunohistochemistry-Paraffin
1:400
Western Blot
1 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lactate Dehydrogenase B/LDHB
Alternate Names
Epididymis Secretory Protein Li 281, HEL-S-281, LDH-H, LDHB, LDHBD, Renal Carcinoma Antigen NY-REN-46, TRG-5
Gene Symbol
LDHB
Additional Lactate Dehydrogenase B/LDHB Products
Product Documents for Lactate Dehydrogenase B Antibody - BSA Free
Product Specific Notices for Lactate Dehydrogenase B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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