Lactyl-Lysine Antibody (PSH03-73)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33149
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Non-species specific
Applications
Dot Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH03-73
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic lactyl lysine-containing peptide.
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Lactyl-Lysine Antibody (PSH03-73)
Flow Cytometry: Lactyl-Lysine Antibody (PSH03-73) [NBP3-33149] -
Flow cytometric analysis of HeLa cells treated with or without 100mM Lactate sodium for 24 hours labeling Lactyl-Lysine.Cells were fixed and permeabilized. Then stained with the primary antibody (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western Blot: Lactyl-Lysine Antibody (PSH03-73) [NBP3-33149] -
Western blot analysis of Lactyl-Lysine on different lysates with Lactyl-Lysine Antibody (PSH03-73) at 1:2,000 dilution.Lane 1: HeLa cell lysateLane 2: HeLa treated with 100mM Lactate sodium for 24 hours cell lysateLysates/proteins at 20 µg/Lane.Observed band size: Mutiple kDaExposure time: 59 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry/ Immunofluorescence: Lactyl-Lysine Antibody (PSH03-73) [NBP3-33149] -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Lactyl-Lysine Antibody (PSH03-73) at 1:1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with The primary antibody at 1:1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Lactyl-Lysine Antibody (PSH03-73)
Application
Recommended Usage
Dot Blot
1:2000
Flow Cytometry
1:1000
Immunocytochemistry/ Immunofluorescence
1:1000
Immunohistochemistry-Paraffin
1:500-1:1000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lactyl-Lysine
Alternate Names
Kla, Lactylated Lysine, Lactyllysine
Additional Lactyl-Lysine Products
Product Documents for Lactyl-Lysine Antibody (PSH03-73)
Product Specific Notices for Lactyl-Lysine Antibody (PSH03-73)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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