LAD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86126

Novus Biologicals, part of Bio-Techne
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NBP1-86126
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LAD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPRTISFRMKPKKENSETTLTRSASMKLPDNTVKLGEKLERYHTAIRRSESVKSRGLPCTELFVAPVGVASKRHLFEKELAGQSRAEPASSRKENLRLSGVVTSRLNLWISRTQESGDQDPQEAQKASSATERTQWGQKSDSSL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LAD1 Antibody

Western Blot: LAD1 Antibody [NBP1-86126]

Western Blot: LAD1 Antibody [NBP1-86126]

Western Blot: LAD1 Antibody [NBP1-86126] - Analysis in human cell lines A-431 and HEK293 using Anti-LAD1 antibody. Corresponding LAD1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: LAD1 Antibody [NBP1-86126]

Immunocytochemistry/ Immunofluorescence: LAD1 Antibody [NBP1-86126]

Immunocytochemistry/Immunofluorescence: LAD1 Antibody [NBP1-86126] - Staining of human cell line MCF7 shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126]

Immunohistochemistry-Paraffin: LAD1 Antibody [NBP1-86126] - Staining of human small intestine shows strong membranous positivity in glandular cells.
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Applications for LAD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAD1

LAD1 is encoded by this gene may be an anchoring filament that is a component of basement membranes. It may contribute to the stability of the association of the epithelial layers with the underlying mesenchyme. [provided by RefSeq]

Alternate Names

LAD, Lad-1, LADA, ladinin 1, ladinin-1, linear IgA bullous dermatosis antigen, Linear IgA disease antigen, linear IgA disease antigen homolog, MGC10355

Gene Symbol

LAD1

Additional LAD1 Products

Product Documents for LAD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LAD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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