Lamin A + C R453W Antibody (12A-2F5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77401
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 12A-2F5
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acids 449-457 of human Lamin A + C
Specificity
Disease-associated point-mutant R453W of Lamin A + C
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Lamin A + C R453W Antibody (12A-2F5)
Western Blot: Lamin A + C R453W Antibody (12A-2F5) [NBP1-77401]
Western Blot: Lamin A + C R453W Antibody (12A-2F5) [NBP1-77401] - Analysis of Lamin A + C R453W in HeLa cells transfected with Flag-tagged Lamin A 1) wild type, 2) R453W and 3) R482W.Applications for Lamin A + C R453W Antibody (12A-2F5)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
1:1000
Application Notes
This Lamin A + C R453W (12A-2F5) point-mutant specific antibody is useful for Western blot, ELISA, Immunoprecipitation and Immunocytochemistry/Immunofluorescence. In WB bands can be seen at 74kDa (Lamin A) and 65 kDa (Lamin C).
Reviewed Applications
Read 1 review rated 4 using NBP1-77401 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lamin A + C R453W
Alternate Names
70 kDa Lamin, CDCD1, CDDC, Charcot Marie Tooth Disease Axonal Type 2B1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, LAMIN A, Lamin A/C, Lamin A/C R453W, LAMIN C, LDP1, LFP, LGMD1B, LMN 1, LMN A, LMN C, LMN1, LMNA, LMNC, PRELAMIN A, PRO1, Progeria 1 (Hutchinson Gilford Type)
Entrez Gene IDs
4000 (Human)
Gene Symbol
LMNA
UniProt
Additional Lamin A + C R453W Products
Product Documents for Lamin A + C R453W Antibody (12A-2F5)
Product Specific Notices for Lamin A + C R453W Antibody (12A-2F5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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