Lamin B1 Antibody (5H8V8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15393

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15393-100ul
NBP3-15393-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5H8V8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lamin B1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 450-550 of human Lamin B1 (P20700). DGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTI

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lamin B1 Antibody (5H8V8)

Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Western Blot: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Western blot analysis of extracts of various cell lines, using Lamin B1 Rabbit mAb (NBP3-15393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded mouse testis using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393]

Immunohistochemistry-Paraffin: Lamin B1 Antibody (5H8V8) [NBP3-15393] - Immunohistochemistry of paraffin-embedded rat spleen using Lamin B1 Rabbit mAb (NBP3-15393) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for Lamin B1 Antibody (5H8V8)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lamin B1

Nuclear lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane.Two main subtypes of nuclear lamins can be distinguished, i.e. A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.

Alternate Names

ADLD, LMN2, LMNB1

Gene Symbol

LMNB1

Additional Lamin B1 Products

Product Documents for Lamin B1 Antibody (5H8V8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lamin B1 Antibody (5H8V8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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