Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44417

Novus Biologicals, part of Bio-Techne
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NBP3-44417
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Catalog #
BSA Free
NBP2-42391
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3118

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Laminin alpha 5 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O15230, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32157808).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Analysis in human placenta and lymph node tissues using NBP3-44417 antibody. Corresponding Laminin alpha 5 RNA-seq data are presented for the same tissues.
Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Staining of human colon shows moderate positivity in basement membrane of glandular cells.
Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Immunohistochemistry-Paraffin: Laminin alpha 5 Antibody [NBP3-44417]

Staining of human kidney shows strong membranous positivity in glomeruli, as well as in basement membrane of renal tubules.
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Applications for Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin alpha 5

Components of the extracellular matrix exert myriad effects on tissues throughout the body. In particular, the laminins, a family of heterotrimeric extracellular glycoproteins, affect tissue development and integrity in such diverse organs as the kidney, lung, skin, and nervous system. It is thought that laminins mediate the attachment, migration, and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Laminins function as heterotrimeric complexes of alpha, beta, and gamma chains, with each chain type representing a different subfamily of proteins. The protein encoded by this gene belongs to the alpha subfamily of laminin chains and is a major component of basement membranes. Two transcript variants encoding different isoforms have been found for this gene, but the full-length nature of one of them has not been determined.

Alternate Names

KIAA0533, KIAA1907, laminin alpha-5 chain, laminin subunit alpha-5, laminin, alpha 5, Laminin-10 subunit alpha, Laminin-11 subunit alpha, Laminin-15 subunit alpha

Gene Symbol

LAMA5

Additional Laminin alpha 5 Products

Product Documents for Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Laminin alpha 5 Antibody (CL3118) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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