Laminin Antibody (4C7)

Novus Biologicals, part of Bio-Techne | Catalog # NB120-17107

Novus Biologicals, part of Bio-Techne
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NB120-17107
NB120-17107-0.1ml
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4C7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Laminin Antibodies »

Product Specifications

Immunogen

Laminin Antibody (4C7) was made to the A chain of human laminin.

Localization

Extracellular. Found in the basement membranes.

Specificity

Laminin Antibody (4C7) reacts with the terminal globular domain of the A chain. The staining with this is sensitive to fixation of paraffin tissues and use of proteolytic enzymatic treatment.

Marker

Basement Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

337 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Laminin Antibody (4C7)

Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107]

Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107]

Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107] - Analysis in formalin fixed paraffin embedded renal carcinoma stained with Laminin Antibody (4C7) [NB120-17107].
Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107]

Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107]

Immunohistochemistry-Paraffin: Laminin Antibody (4C7) [NB120-17107] - Analysis in formalin fixed paraffin embedded renal carcinoma with Laminin Antibody (4C7) [NB120-17107].

Applications for Laminin Antibody (4C7)

Application
Recommended Usage

Immunohistochemistry

1:5-1:20

Immunohistochemistry-Paraffin

1:5-1:20
Application Notes
IHC-P: recommended pretreatment of citrate buffer. Recommended incubation time of 30 min at RT. Use in Western blot reported in scientific literature (PMID: 20671748).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Laminin

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).

References

1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y., . . . Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5

Alternate Names

LAMA, Laminin A chain, laminin subunit alpha-1, laminin, alpha 1, Laminin-1 subunit alpha, Laminin-3 subunit alpha, PTBHS, S-LAM alpha, S-laminin subunit alpha

Entrez Gene IDs

284217 (Human); 16772 (Mouse); 316758 (Rat)

Gene Symbol

LAMA1

Additional Laminin Products

Product Documents for Laminin Antibody (4C7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Laminin Antibody (4C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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