Laminin beta 1 Antibody (CL2970)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42385

Novus Biologicals, part of Bio-Techne
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NBP2-42385
NBP2-42385-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2970

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin beta 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GPRCDKCTRGYSGVFPDCTPCHQCFALWDVIIAELTNRTHRFLEKAKALKISGVIGPYRETVDSVERKVSEIKDILAQSPAAEPLKNIGNLFEEAEKLIKDVTEMMAQVEVKLSDTTSQSNSTAKELDSLQTEAESLDNTVKELAEQLEF

Reactivity Notes

Expected species cross reactivity based on sequence identity: Rat (89%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin beta 1 Antibody (CL2970)

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis of purified human recombinant Laminin-111, Laminin-211, Laminin-411, Laminin-511, Laminin-521 and Laminin-332.
Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Staining of human lymph node shows absence of immunoreactivity in lymphoid cells (negative control).
Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385]

Western Blot: Laminin beta 1 Antibody (CL2970) [NBP2-42385] - Analysis in human placenta tissue.
View 4 more images

Applications for Laminin beta 1 Antibody (CL2970)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin beta 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

CLM, cutis laxa with marfanoid phenotype, Laminin B1 chain, laminin subunit beta-1, laminin, beta 1, Laminin-1 subunit beta, Laminin-10 subunit beta, Laminin-12 subunit beta, Laminin-2 subunit beta, Laminin-6 subunit beta, Laminin-8 subunit beta, MGC142015

Gene Symbol

LAMB1

UniProt

P07942 (Human)

Additional Laminin beta 1 Products

Product Documents for Laminin beta 1 Antibody (CL2970)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin beta 1 Antibody (CL2970)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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