Laminin gamma 1 Antibody (A5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47842
![Immunocytochemistry/ Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]](https://resources.bio-techne.com/images/products/Laminin-gamma-1-Antibody-A5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47842-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # A5
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Laminin gamma 1 Antibody (A5) - Azide and BSA Free
Immunogen
Murine EHS laminin preparation
Reactivity Notes
Bovine reported by a verified customer review.
Localization
Basement membrane
Specificity
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This monoclonal antibody reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific monoclonal antibodys. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This monoclonal antibody stains uniformly all human and murine basement membranes.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44751).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Laminin gamma 1 Antibody (A5) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]
Immunocytochemistry/Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue). Image from verified customer review.![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]](https://resources.bio-techne.com/images/products/Laminin-gamma-1-Antibody-A5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47842-img0002.jpg "Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]")
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842]
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).Applications for Laminin gamma 1 Antibody (A5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Laminin gamma 1
Additional Laminin gamma 1 Products
Product Documents for Laminin gamma 1 Antibody (A5) - Azide and BSA Free
Product Specific Notices for Laminin gamma 1 Antibody (A5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...