Laminin gamma 1 Antibody (CL3196)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42395

Novus Biologicals, part of Bio-Techne
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NBP2-42395
NBP2-42395-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Predicted:

Mouse (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL3196

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Laminin gamma 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKVADHRVKLQELESLIANLGTGDEMVTDQAFEDRLKEAEREVMDLLREAQDVKDVDQNLMDRLQRVNNTLSSQISRLQNIRNTIEETGNLAEQARAHVENTERLIEI

Epitope

PACYRLVKDKVADHR

Reactivity Notes

Rat reactivity reported in the scientific literature (PMID: 30044554). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Laminin gamma 1 Antibody (CL3196)

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Analysis of purified human recombinant Laminin-111, Laminin-121, Laminin-211, protein Laminin-221, Laminin-411, Laminin-421, Laminin-511, Laminin-521 and Laminin-332.
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Staining of human tonsil shows absence of immunoreactivity in lymphoid tissue (negative control).
Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395]

Western Blot: Laminin gamma 1 Antibody (CL3196) [NBP2-42395] - Analysis in human lung tissue.
View 4 more images

Applications for Laminin gamma 1 Antibody (CL3196)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry/Immunofluorescence reported in scientific literature (PMID:30044554).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Laminin gamma 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

LAMC1, Laminin B2

Gene Symbol

LAMC1

UniProt

P11047 (Human)

Additional Laminin gamma 1 Products

Product Documents for Laminin gamma 1 Antibody (CL3196)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Laminin gamma 1 Antibody (CL3196)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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