Laminin gamma 1 Antibody (JE40-75)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32522
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE40-75
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Laminin gamma 1 aa 21-70 / 1,609. (Uniprot: P11047)
Localization
Secreted, extracellular space, extracellular matrix, basement membrane.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
178 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Laminin gamma 1 Antibody (JE40-75)
Western Blot: Laminin gamma 1 Antibody (JE40-75) [NBP3-32522]
Western Blot: Laminin gamma 1 Antibody (JE40-75) [NBP3-32522] - Western blot analysis of Laminin gamma 1 on different lysates with Rabbit anti-Laminin gamma 1 antibody (NBP3-32522) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: OVCAR-3 cell lysate
Lane 4: A549 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 178 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32522) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Laminin gamma 1 Antibody (JE40-75)
Application
Recommended Usage
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Laminin gamma 1
Alternate Names
LAMC1, Laminin B2
Gene Symbol
LAMC1
Additional Laminin gamma 1 Products
Product Documents for Laminin gamma 1 Antibody (JE40-75)
Product Specific Notices for Laminin gamma 1 Antibody (JE40-75)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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