Laminin gamma 1 Antibody (SPM193)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44752

Novus Biologicals, part of Bio-Techne
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NBP2-44752-0.02mg
NBP2-44752-0.1mg
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2a Kappa Clone # SPM193

Concentration

0.2 mg/ml

View all Laminin gamma 1 Antibodies »

Product Specifications

Immunogen

Murine EHS laminin preparation

Localization

Basement membrane

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Theoretical MW

210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-11464)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Laminin gamma 1 Antibody (SPM193)

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (SPM193) [NBP2-44752]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (SPM193) [NBP2-44752]

Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (SPM193) [NBP2-44752] - Human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (SPM193).

Applications for Laminin gamma 1 Antibody (SPM193)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Laminin gamma 1

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Alternate Names

LAMC1, Laminin B2

Gene Symbol

LAMC1

UniProt

P11047 (Human), Q5VYE7 (Human), Q6NVY8 (Human)

Additional Laminin gamma 1 Products

Product Documents for Laminin gamma 1 Antibody (SPM193)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Laminin gamma 1 Antibody (SPM193)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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