Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all LAMP-1/CD107a Antibodies »

Product Specifications

Immunogen

This antibody was generated with a synthetic peptide corresponding to amino acid residues 405-416 of human LAMP1 with N-terminal added cysteine-glycine, conjugated to KLH.

Reactivity Notes

The antigen sequence is identical in rat and mouse. Rabbit reactivity reported by customer review.

Marker

Late Endosome Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LAMP-1/CD107a Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956]

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956]

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Human HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 5 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956]

Immunocytochemistry/ Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956]

Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody - BSA Free [NB600-956] - Rat NRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% Saponin. Fixed cells were stained with 10 ug/mL Anti-LAMP1 and developed with Goat Anti-Rabbit IgG, FITC conjugate.

Applications for LAMP-1/CD107a Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5-10 ug/ml
Application Notes
Useful for indirect IF using human HeLa, rat NRK, and mouse NIH3T3 cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NB600-956 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAMP-1/CD107a

LAMP-1 (lysosome-associated membrane protein 1), also known as CD107a (cluster of differentiation 107a), is a major component of lysosomal membranes that plays an important role in lysosomal biogenesis, autophagy, and cholesterol metabolism (1). LAMP-1 is a type I transmembrane glycoprotein that is expressed on plasma membranes and the membranes of endosomes, autolysosomes, and lysosomes (1,2). Additionally, LAMP-1/CD107a is a commonly used marker for natural killer (NK) cell degranulation (3). LAMP-1 and another lysosomal-associated membrane protein, LAMP-2, together make up about half of all lysosome membrane proteins (1). Additionally, LAMP-1 has a role in presenting carbohydrate ligands to selectins (2). Human LAMP-1 protein is comprised of 417 amino acids (aa) with a theoretical molecular weight of 44.8 kDa; however, glycosylation can increase the molecular weight upwards of 120 kDa (1, 4). Structurally, LAMP-1 protein contains a large luminal/extracellular domain (29-382 aa), a helical transmembrane domain (383-405 aa), and a short cytoplasmic tail (406-417 aa) (1,2). Additionally, the protein has many N- and O-linked glycosylation sites which helps with stability in the membrane (1,2).

LAMP-1 plays an important role in autophagy-mediated ATP-release during apoptosis where lysosomes containing intracellular ATP migrate to the plasma membrane and, during exocytosis, LAMP-1 is exposed to the cell surface (5). Studies have found that knockdown of LAMP-1 blocks the ATP release from the cell (5). Furthermore, an absence of LAMP-1 and LAMP-2 leads to an accumulation of lysosomal cholesterol (6). Lysosomal membrane dysfunction or defects has also been associated with disease development (6,7). For example, one feature of pancreatitis is autophagy impairment which is caused by lysosomal dysfunction and a corresponding decrease in lysosomal-membrane associated proteins LAMP-1 and LAMP-2 (7).

References

1. Eskelinen E. L. (2006). Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Molecular aspects of medicine, 27(5-6), 495-502. https://doi.org/10.1016/j.mam.2006.08.005

2. Cheng, X. T., Xie, Y. X., Zhou, B., Huang, N., Farfel-Becker, T., & Sheng, Z. H. (2018). Revisiting LAMP1 as a marker for degradative autophagy-lysosomal organelles in the nervous system. Autophagy, 14(8), 1472-1474. https://doi.org/10.1080/15548627.2018.1482147

3. Krzewski, K., & Coligan, J. E. (2012). Human NK cell lytic granules and regulation of their exocytosis. Frontiers in immunology, 3, 335. https://doi.org/10.3389/fimmu.2012.00335

4. Uniprot (P11279)

5. Wang, Y., Martins, I., Ma, Y., Kepp, O., Galluzzi, L., & Kroemer, G. (2013). Autophagy-dependent ATP release from dying cells via lysosomal exocytosis. Autophagy, 9(10), 1624-1625. https://doi.org/10.4161/auto.25873

6. Schwake, M., Schr0der, B., & Saftig, P. (2013). Lysosomal membrane proteins and their central role in physiology. Traffic (Copenhagen, Denmark), 14(7), 739-748. https://doi.org/10.1111/tra.12056

7. Gukovsky, I., Pandol, S. J., Mareninova, O. A., Shalbueva, N., Jia, W., & Gukovskaya, A. S. (2012). Impaired autophagy and organellar dysfunction in pancreatitis. Journal of gastroenterology and hepatology, 27 Suppl 2(Suppl 2), 27-32. https://doi.org/10.1111/j.1440-1746.2011.07004.x

Long Name

Lysosome-associated Membrane Glycoprotein 1

Alternate Names

CD107a, LAMP1

Entrez Gene IDs

3916 (Human); 239739 (Mouse); 25328 (Rat)

Gene Symbol

LAMP1

UniProt

P11279

Additional LAMP-1/CD107a Products

Product Documents for LAMP-1/CD107a Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LAMP-1/CD107a Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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