LANA2 Antibody (CM-A807) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB200-167

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Virus

Cited:

Human, Virus

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CM-A807

Format

BSA Free

Concentration

2.0 mg/ml

View all LANA2 Antibodies »

Product Specifications

Immunogen

Recombinant LANA2 fusion protein.

Reactivity Notes

Reacts with viral LANA2 protein and shows no cross-reactivity to cellular proteins. Also reacts with human and mouse viral KSHV proteins.

Localization

Nuclear

Specificity

This is specific for KSHV LANA2 (residues 143-310 epitope recognition).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for LANA2 Antibody (CM-A807) - BSA Free

Western Blot: LANA2 Antibody (CM-A807)BSA Free [NB200-167]

Western Blot: LANA2 Antibody (CM-A807)BSA Free [NB200-167]

Western Blot: LANA2 Antibody (CM-A807) [NB200-167] - Detection of LANA2 in infected lysates (30 ug) using 2 ug/ml of NB 200-167. ECL detection 1.5 minutes. Lane 1: BCBL-1 (KSHV+) Lane 2: BC-1 (KSHV+/EBV+) Lane 3: BCP-1 (KSHV+)
Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) - BSA Free [NB200-167]

Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) - BSA Free [NB200-167]

Immunohistochemistry-Paraffin: LANA2 Antibody (CM-A807) [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource and performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA. Representative image shows the LANA staining of skin KS lesion. Image collected and cropped by CiteAb from the following publication (https://mbio.asm.org/lookup/doi/10.1128/mBio.02217-17), licensed under a CC-BY license.
LANA2 Antibody (CM-A807) - BSA Free

Immunohistochemistry: LANA2 Antibody (CM-A807) - BSA Free [NB200-167] -

Immunohistochemistry: LANA2 Antibody (CM-A807) - BSA Free [NB200-167] - vIRF3 expression in KS tissues. We obtained KS tissue microarrays from AIDS Cancer Specimen Resource & performed immunohistochemistry, visualized by Aperio F.L. digital pathological scanning. A biopsy sample of KS TMA was stained with anti-LANA & anti-vIRF3 (CM-A807). Representative images show the LANA staining of skin KS lesion & the vIRF3 staining of skin, tonsil, & mouth KS lesions & positive staining corresponding to either LANA or vIRF3 in total KS biopsy specimens embedded in TMA. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29339432), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for LANA2 Antibody (CM-A807) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Knockout Validated

reported in scientific literature (Golas et al)

Western Blot

2-4 ug/ml
Application Notes
By Western Blot, this antibody recognizes a viral protein in KSHV infected B-cells (~66-70 kDa).

Reviewed Applications

Read 1 review rated 5 using NB200-167 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LANA2

LANA2 (latency-associated nuclear antigen 2; also known as viral interferon regulatory factor 3) is a Kaposi's sarcoma-associated herpesvirus (KSHV) latent protein which is encoded by ORFK10.5 and is exclusively expressed by virus infected B- cells. LANA2 has been shown to be critical in KSHV-mediated tumorigenesis as it inhibits p53 function, dsRNA-activated protein kinase R (PKR)-dependent apoptosis, nuclear factor kB activity, interferon regulatory factor 7-mediated interferon signal transduction and virus-mediated transcriptional activation of the alpha-interferon promoter. LANA2 stabilizes and triggers the induction of HIF-1alpha's transcriptional activity and disrupts PODs (PML oncogenic domains). Moreover, LANA2 has been shown to be required for the survival of primary effusion lymphoma (PEL) cells infected with KSHV alone or dually infected by Epstein-Barr virus (EBV) and KSHV, wherein it shows nuclear and cytoplasmic expression.

Alternate Names

LANA 2, LANA-2, Latency-associated nuclear antigen 2, vIRF 3, vIRF3, vIRF-3

Additional LANA2 Products

Product Documents for LANA2 Antibody (CM-A807) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LANA2 Antibody (CM-A807) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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