Langerin/CD207 Antibody (929F3.01)

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100]

Immunocytochemistry/Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Mouse epidermal sheet.

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Analysis using the HRP conjugate of DDX0362P-100. Staining of human skin frozen section.

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100]

Immunohistochemistry-Frozen: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - IHC staining of human skin cryosections with 929F3-HRP

Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - LC migration from the epidermis is reduced the in K14E7 mice.C57Bl/6 & K14E7 mice were painted with FITC on a 15mm2 area of abdomen & inguinal lymph nodes harvested 48 h later. Single cell suspensions were stained with CD11c, MHC class II & CD207 (A). The absolute number of FITC positive cells was determined (B). The total migrated LC in the lymph nodes as a percentage of the mean total number of LC in the painted skin is shown (C). *P < 0.05 (Mann-Whitney U test). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Flow Cytometry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Antigen uptake is reduced in K14E7 mice.Epidermal cell suspensions from C57BL/6 & K14E7 mice were cultured with Alexa-555-labelled OVA overnight at 37°C, stained & analysed by flow cytometry (A). The Alexa-555-OVA+ LC (CD45.2+, MHC Class II+, CD207+) as a percentage of total LC (B) & the level of expression of Alexa-555-OVA on the positive stained LC is shown (C). **P < 0.01; *P < 0.05 (Mann-Whitney U test). Confocal imaging of CD207+, MHCII+ LC that have taken up Alexa-555-OVA (D). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - Antigen uptake is reduced in K14E7 mice.Epidermal cell suspensions from C57BL/6 & K14E7 mice were cultured with Alexa-555-labelled OVA overnight at 37°C, stained & analysed by flow cytometry (A). The Alexa-555-OVA+ LC (CD45.2+, MHC Class II+, CD207+) as a percentage of total LC (B) & the level of expression of Alexa-555-OVA on the positive stained LC is shown (C). **P < 0.01; *P < 0.05 (Mann-Whitney U test). Confocal imaging of CD207+, MHCII+ LC that have taken up Alexa-555-OVA (D). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Immunohistochemistry: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - The absolute number of LC is increased in K14E7 mouse epidermis.Sections of C57Bl/6 (A) & K14E7 (B) mouse ear were stained with CD207 & imaged by epifluorescence microscopy. The epidermis (top) is indicated by a broad dashed line, & the epidermis/dermis interface by a narrow dashed line; scale bar = 50μm. LC (CD45.2, CD11c, CD207, MHCII+) harvested from a 5.5 mm diameter sample of dorsal ear epidermis were enumerated, with counting beads, by flow cytometry. The absolute number (C) & the percentage of LC (D) are shown. Confocal imaging was carried out on CD207-stained sheets of dorsal ear skin (E). Scale bar represents 20μm. Dendrites on the CD207+ LC were enumerated following confocal imaging (F). **P < 0.01 (Mann-Whitney U test). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - The absolute number of LC is increased in K14E7 mouse epidermis.Sections of C57Bl/6 (A) & K14E7 (B) mouse ear were stained with CD207 & imaged by epifluorescence microscopy. The epidermis (top) is indicated by a broad dashed line, & the epidermis/dermis interface by a narrow dashed line; scale bar = 50μm. LC (CD45.2, CD11c, CD207, MHCII+) harvested from a 5.5 mm diameter sample of dorsal ear epidermis were enumerated, with counting beads, by flow cytometry. The absolute number (C) & the percentage of LC (D) are shown. Confocal imaging was carried out on CD207-stained sheets of dorsal ear skin (E). Scale bar represents 20μm. Dendrites on the CD207+ LC were enumerated following confocal imaging (F). **P < 0.01 (Mann-Whitney U test). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] -

Immunocytochemistry/ Immunofluorescence: Langerin/CD207 Antibody (929F3.01) [DDX0362P-100] - The absolute number of LC is increased in K14E7 mouse epidermis.Sections of C57Bl/6 (A) & K14E7 (B) mouse ear were stained with CD207 & imaged by epifluorescence microscopy. The epidermis (top) is indicated by a broad dashed line, & the epidermis/dermis interface by a narrow dashed line; scale bar = 50μm. LC (CD45.2, CD11c, CD207, MHCII+) harvested from a 5.5 mm diameter sample of dorsal ear epidermis were enumerated, with counting beads, by flow cytometry. The absolute number (C) & the percentage of LC (D) are shown. Confocal imaging was carried out on CD207-stained sheets of dorsal ear skin (E). Scale bar represents 20μm. Dendrites on the CD207+ LC were enumerated following confocal imaging (F). **P < 0.01 (Mann-Whitney U test). Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0127155), licensed under a CC-BY license. Not internally tested by Novus Biologicals.