Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # S165-38

Concentration

1 mg/ml

View all LAR Antibodies »

Product Summary for LAR/PTPRF Antibody (S165-38)

Immunogen

Fusion protein amino acids 1315-1607 (cytoplasmic C-terminus) of human LAR. 97% identical in both rat and mouse. >80% identity with PTPRD and PTPRS. >50% identity with PTPRM and PTPRK.

Localization

Membrane

Specificity

Greater then 80% identity with PTPRD and PTPRS. Greater then 50% identity with PTPRM and PTPRK.Detects approx 85kDa (full length protein is 210 kDa -smaller due to proteolysis into P-subunit containing transmembrane and intracellular domains.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for LAR/PTPRF Antibody (S165-38)

Western Blot: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Western Blot: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Western Blot: LAR/PTPRF Antibody (S165-38) [NBP2-42172] - Western Blot analysis of Rat Brain Membrane showing detection of LAR/PTPRF protein using Mouse Anti-LAR/PTPRF Monoclonal Antibody, Clone S165-38 (NBP2-42172). Primary Antibody: Mouse Anti-LAR/PTPRF Monoclonal Antibody (NBP2-42172) at 1:250.
Immunocytochemistry/ Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Immunocytochemistry/ Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Immunocytochemistry/Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-LAR/PTPRF/PTPRF Monoclonal Antibody, Clone S165-38 (NBP2-42172). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-LAR/PTPRF/PTPRF Monoclonal Antibody (NBP2-42172) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) LAR/PTPRF/PTPRF Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Immunocytochemistry/ Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172]

Immunocytochemistry/Immunofluorescence: LAR/PTPRF Antibody (S165-38) [NBP2-42172] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LAR/PTPRF Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane. Magnification: 60X.
View 1 more images

Applications for LAR/PTPRF Antibody (S165-38)

Application
Recommended Usage

Western Blot

1:1000
Application Notes
1 ug/ml of LAR/PTPRF Antibody was sufficient for detection of LAR/PTPRF in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-42172 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LAR

PTPRF, also known as Receptor-type tyrosine-protein phosphatase F, is a 213kDa 1907 amino acid protein with a shorter 212kDa 1898 isoform. PTPRF is a member of the protein tyrosine phosphatase (PTP) family and functions as a possible cell adhesion receptor. Research is being conducted on PTPRF in relation to melanoma, carcinoma, retinitis, thyroid carcinoma, ureterocele, lung cancer, breast cancer and obesity. PTPRF is linked to the cell adhesion and insulin signaling pathways where it interacts with CAV1, PRPF40A, SKIL, ZNF512B and JUP.

Long Name

Leukocyte Antigen-Related Tyrosine Phosphatase

Alternate Names

PTPRF

Gene Symbol

PTPRF

UniProt

P10586 (Human)

Additional LAR Products

Product Documents for LAR/PTPRF Antibody (S165-38)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LAR/PTPRF Antibody (S165-38)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.