Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 33D196

Format

BSA Free

Concentration

1.0 mg/ml

View all Lck Antibodies »

Product Specifications

Immunogen

This monoclonal antibody was raised against a synthetic peptide containing amino acid residues of human lck protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Lck Antibody (33D196) - BSA Free

Western Blot: Lck Antibody (33D196)BSA Free [NB100-56678]

Western Blot: Lck Antibody (33D196)BSA Free [NB100-56678]

Western Blot: Lck Antibody (33D196) [NB100-56678] - Analysis for Lck using NB100-56678 at 1:1000 dilution against 20 ug/lane of Jurkat cell lysate.

Applications for Lck Antibody (33D196) - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lck

Lck is a lymphoid-specific cytosolic protein tyrosine kinase (PTK), which is essential for T-cell development and function. It is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated Lck to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAMs) in the cytoplasmic tails of the TCR-chains and CD3 subunits. The phospho-ITAMs serve as docking sites for Src homology domain 2 (SH2)-containing molecules, predominantly ZAP-70 and Syk. Only then can ZAP-70 undergo tyrosine phosphorylation, become enzymatically active and further phosphorylate downstream effector molecules. In addition, Lck contributes to signaling by other receptor molecules. Lck is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature TCR.

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Entrez Gene IDs

3932 (Human); 16818 (Mouse)

Gene Symbol

LCK

UniProt

P06239

Additional Lck Products

Product Documents for Lck Antibody (33D196) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Lck Antibody (33D196) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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