Lck Antibody (LCK-01) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-336

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # LCK-01

Format

BSA Free

Concentration

1.0 mg/ml

View all Lck Antibodies »

Product Specifications

Immunogen

Peptide corresponding to amino acids 22-36 in the sequence of human Lck.

Localization

Cytoplasmic

Specificity

The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Lck Antibody (LCK-01) - BSA Free

Western Blot: Lck Antibody (LCK-01)BSA Free [NB500-336]

Western Blot: Lck Antibody (LCK-01)BSA Free [NB500-336]

Western Blot: Lck Antibody (LCK-01) [NB500-336] - Fig. 1. Western Blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck Lane 2: HEK-293T cell line transfected with Lck Lane 3: HEK-293T cell line (non-transfected).
Flow Cytometry: Lck Antibody (LCK-01) - BSA Free [NB500-336]

Flow Cytometry: Lck Antibody (LCK-01) - BSA Free [NB500-336]

Flow Cytometry: Lck Antibody (LCK-01) [NB500-336] - Staining of Lck in human peripheral blood cells using anti-Lck (LCK-01) Alexa Fluor® 647.

Applications for Lck Antibody (LCK-01) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Flow Cytometry

1-5 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Lck

Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in its zeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by Lck and thereby activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to the plasma membrane, there is also a significant fraction associated with the Golgi apparatus, which may contribute to Raf activation under conditions of weak stimulation through the TCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors.

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Entrez Gene IDs

3932 (Human); 16818 (Mouse)

Gene Symbol

LCK

Additional Lck Products

Product Documents for Lck Antibody (LCK-01) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lck Antibody (LCK-01) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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