Lck [p Tyr394] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13305

Novus Biologicals, part of Bio-Techne
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NBP3-13305
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Lck Antibodies »

Product Summary for Lck [p Tyr394] Antibody

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Tyr394 of human Lck. The exact sequence is proprietary.

Modification

p Tyr 394

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Lck [p Tyr394] Antibody

Western Blot: Lck [p Tyr394] Antibody [NBP3-13305]

Western Blot: Lck [p Tyr394] Antibody [NBP3-13305]

Western Blot: Lck [p Tyr394] Antibody [NBP3-13305] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Lck (phospho Tyr394) antibody (NBP3-13305) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: Lck [p Tyr394] Antibody [NBP3-13305]

Western Blot: Lck [p Tyr394] Antibody [NBP3-13305]

Western Blot: Lck [p Tyr394] Antibody [NBP3-13305] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lck (phospho Tyr394) antibody (NBP3-13305) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for Lck [p Tyr394] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Lck

The human T-cell or lymphocyte specific gene Lck/p56 is a member of the Src family of non-receptors tyrosine kinase. Mostly expressed in T cells, Lck has been shown to be critical for the normal development of T lymphocytes (1). Additionally, the N-terminal region of the tyrosine kinase Lck interacts with the cytoplasmic domains of CD4 and CD8 (2). Autophosphorylation at Tyr-394 appears to be required for maximum catalytic activity (3) but it can also be suppressed by phosphorylation of the carboxyl-terminal tyrosine Tyr-505 (4).

Long Name

Lymphocyte Protein Tyrosine Kinase

Alternate Names

Hck-3, Lcktkr, LSTRA, p56lck, pp58lck, YT16

Gene Symbol

LCK

Additional Lck Products

Product Documents for Lck [p Tyr394] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lck [p Tyr394] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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