LDB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31362

Novus Biologicals, part of Bio-Techne
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NBP1-31362
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (97%), Monkey (100%), Porcine (98%), Xenopus (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LDB1 Antibodies »

Product Summary for LDB1 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (97%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LDB1 Antibody

Knockout Validated: LDB1 Antibody [NBP1-31362]

Knockout Validated: LDB1 Antibody [NBP1-31362]

Knockout Validated: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-31362]

Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-31362]

Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362]

Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.
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Applications for LDB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LDB1

LDB1 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neuron

Alternate Names

carboxy terminal LIM domain protein 2, Carboxyl-terminal LIM domain-binding protein 2, CLIM-2, CLIM2hLdb1, LDB-1, LIM domain binding 1, LIM domain-binding factor-1, LIM domain-binding protein 1, NLILIM domain-binding factor CLIM2, Nuclear LIM interactor

Gene Symbol

LDB1

Additional LDB1 Products

Product Documents for LDB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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