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LDB3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2445

Novus Biologicals, part of Bio-Techne
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NB100-2445

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-KSQNKPEDEADE corresponding to internal region according to NP_009009.1, NP_001073583.1, NP_001073584.1, NP_001073585.1.

Epitope

C-KSQNKPEDEADE

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LDB3 Antibody

Western Blot: LDB3 Antibody [NB100-2445]

Western Blot: LDB3 Antibody [NB100-2445]

Western Blot: LDB3 Antibody [NB100-2445] - Staining of Human Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
LDB3 Antibody

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] -

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - Negative Control showing staining of paraffin embedded Human Heart, with no primary antibody.
LDB3 Antibody

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] -

Immunohistochemistry-Paraffin: LDB3 Antibody [NB100-2445] - (6ug/ml) staining of paraffin embedded Human Heart. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for LDB3 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

6-7 ug/ml

Peptide ELISA

Detection limit 1:1000

Western Blot

0.1 - 1 ug/ml
Application Notes
WB: WB experiments gave bands at approx. 90 kDa band and 35 kDa band in human skeletal muscle lysate after 1 ug/ml antibody staining. These bands correspond to earlier findings are reported in scientific literature with different antibodies (PMID: 12499364). This protein has a calculated MW of 77.1 kDa band according to NP_009009.1.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LDB3

Gene ID(s): 11155 (human)

Alternate Names

CMD1C, CYPHER, KIAA01613, KIAA0613FLJ35865, LDB3Z1, LDB3Z4, LIM domain binding 3, LIM domain-binding protein 3, ORACLE, PDLIM6, PDZ and LIM domain 6, Protein cypher, ZASPLVNC3, Z-band alternatively spliced PDZ-motif protein

Entrez Gene IDs

11155 (Human)

Gene Symbol

LDB3

Additional LDB3 Products

Product Documents for LDB3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LDB3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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