LEDGF Antibody (PSH02-55)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32860
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # PSH02-55
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant protein within human LEDGF aa 51-530 / 530 (O75475). (Uniprot: O75475)
Localization
Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for LEDGF Antibody (PSH02-55)
Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860]
Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution.Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: 293T cell lysate
Lane 4: SH-SY5Y cell lysate
Lane 5: THP-1 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 ug/Lane.
Predicted band size: 60 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860]
Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860]
Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Applications for LEDGF Antibody (PSH02-55)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry-Paraffin
1:1000
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: LEDGF
Long Name
Lens Epithelium-derived Growth Factor
Alternate Names
CLL-associated antigen KW-7, DFS 70, PSIP1, Transcriptional coactivator p75/p52
Gene Symbol
PSIP1
Additional LEDGF Products
Product Documents for LEDGF Antibody (PSH02-55)
Product Specific Notices for LEDGF Antibody (PSH02-55)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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