LEF1 Antibody (9I9T4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16420

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16420-100ul
NBP3-16420-20ul
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9I9T4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LEF1 Antibodies »

Product Specifications

Immunogen

Recombinant protein fo a sequence within amino acids 50-150 of human LEF1 (Q9UJU2). LADIKSSLVNESEIIPASNGHEVARQAQTSQEPYHDKAREHPDDGKHPDGGLYNKGPSYSSYSGYIMMPNMNNDPYMSNGSLSPPIPRTSNKVPVVQPSHA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LEF1 Antibody (9I9T4)

Western Blot: LEF1 Antibody (9I9T4) [NBP3-16420]

Western Blot: LEF1 Antibody (9I9T4) [NBP3-16420]

Western Blot: LEF1 Antibody (9I9T4) [NBP3-16420] - Western blot analysis of extracts of various cell lines, using LEF1 Rabbit mAb (NBP3-16420) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for LEF1 Antibody (9I9T4)

Application
Recommended Usage

Immunoprecipitation

1:1000 - 1:2000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LEF1

Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]

Long Name

Lymphoid Enhancer-binding Factor 1

Alternate Names

TCF10, TCF1ALPHA, TCF7L3

Gene Symbol

LEF1

Additional LEF1 Products

Product Documents for LEF1 Antibody (9I9T4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LEF1 Antibody (9I9T4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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