LEF1 Antibody (LEF1/341R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08435
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]](https://resources.bio-techne.com/images/products/LEF1-Antibody-LEF1-341R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08435-img0002.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # LEF1/341R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa100-200) of human LEF1 protein (exact sequence is proprietary) (Uniprot: Q9UJU2)
Localization
Nucleus.
Specificity
Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor that belongs to the TCF/LEF family. LEF1 participates as a regulator in Wnt signaling pathways. LEF1 is an important factor in lymphopoiesis and is expressed normally in T and pro-B cells but not expressed in mature B cells. Anti-LEF1 may be used as an aid for differentiation of chronic lymphocytic leukemia/small lymphocytic lymphoma from other small B cell lymphomas.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07769).
Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for LEF1 Antibody (LEF1/341R) - Azide and BSA Free
Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]
Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435] - IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.![Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]](https://resources.bio-techne.com/images/products/LEF1-Antibody-LEF1-341R-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08435-img0001.jpg "Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]")
Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435]
Immunohistochemistry-Paraffin: LEF1 Antibody (LEF1/341R) - Azide and BSA Free [NBP3-08435] - IHC analysis of formalin-fixed, paraffin-embedded human lymph node. Strong nuclear staining of non-germinal center cells using LEF1( LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.
LEF1 Antibody (LEF1/341R)
Formalin-fixed, paraffin-embedded analysis of human lymph node. Strong nuclear staining of non-germinal center cells using LEF1 antibody (LEF1/341R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for LEF1 Antibody (LEF1/341R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: LEF1
Long Name
Lymphoid Enhancer-binding Factor 1
Alternate Names
TCF10, TCF1ALPHA, TCF7L3
Gene Symbol
LEF1
Additional LEF1 Products
Product Documents for LEF1 Antibody (LEF1/341R) - Azide and BSA Free
Product Specific Notices for LEF1 Antibody (LEF1/341R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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