LEMD3 Antibody (OTI6C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02211

Novus Biologicals, part of Bio-Techne
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NBP2-02211
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6C10

Concentration

0.56 mg/ml

View all LEMD3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LEMD3 Antibody (OTI6C10)

Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Western Blot: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Analysis of extracts (35ug) from 9 different cell lines by using anti-LEMD3 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Immunocytochemistry/Immunofluorescence: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.
Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211]

Immunohistochemistry-Paraffin: LEMD3 Antibody (OTI6C10) [NBP2-02211] - Staining of paraffin-embedded liver tissue using anti-LEMD3mouse monoclonal antibody.
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Applications for LEMD3 Antibody (OTI6C10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2-4ug/mg

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.56 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LEMD3

This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis

Alternate Names

inner nuclear membrane protein Man1, integral inner nuclear membrane protein, LEM domain containing 3, MAN1LEM domain-containing protein 3

Entrez Gene IDs

495 (Human)

Gene Symbol

ATP4A

Additional LEMD3 Products

Product Documents for LEMD3 Antibody (OTI6C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for LEMD3 Antibody (OTI6C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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