LEMD3 Antibody (OTI6C10) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71744

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6C10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all LEMD3 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human LEMD3 (NP_055134) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LEMD3 Antibody (OTI6C10) - Azide and BSA Free

Western Blot: LEMD3 Antibody (OTI6C10)Azide and BSA Free [NBP2-71744]

Western Blot: LEMD3 Antibody (OTI6C10)Azide and BSA Free [NBP2-71744]

Western Blot: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEMD3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEMD3.
Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744]

Immunocytochemistry/ Immunofluorescence: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744]

Immunocytochemistry/Immunofluorescence: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY LEMD3.
Immunohistochemistry: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744]

Immunohistochemistry: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744]

Immunohistochemistry: LEMD3 Antibody (OTI6C10) - Azide and BSA Free [NBP2-71744] - Staining of paraffin-embedded liver tissue using anti-LEMD3mouse monoclonal antibody.
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Applications for LEMD3 Antibody (OTI6C10) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

2-4ug/mg

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LEMD3

The MAN antigens, such as MAN1, are nuclear membrane proteins that are recognized by autoantibodies from a patient with a collagen vascular disease. Because MAN1 contains an LEM domain, the gene is also called LEMD3 (for 'LEM domain-containing-3').[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Alternate Names

inner nuclear membrane protein Man1, integral inner nuclear membrane protein, LEM domain containing 3, MAN1LEM domain-containing protein 3

Gene Symbol

LEMD3

Additional LEMD3 Products

Product Documents for LEMD3 Antibody (OTI6C10) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LEMD3 Antibody (OTI6C10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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