Leptin/OB Antibody (7E10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59497

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 7E10

Format

BSA Free

Concentration

1 mg/ml

View all Leptin/OB Antibodies »

Product Specifications

Immunogen

Recombinant human Leptin/OB (22-167aa) purified from E. coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Leptin/OB Antibody (7E10) - BSA Free

Western Blot: Leptin/OB Antibody (7E10)BSA Free [NBP2-59497]

Western Blot: Leptin/OB Antibody (7E10)BSA Free [NBP2-59497]

Western Blot: Leptin/OB Antibody (7E10) [NBP2-59497] - Lane 1: 293T cell lysates, Lane 2: Leptin transfected 293T cell lysates
Immunocytochemistry/ Immunofluorescence: Leptin/OB Antibody (7E10) - BSA Free [NBP2-59497]

Immunocytochemistry/ Immunofluorescence: Leptin/OB Antibody (7E10) - BSA Free [NBP2-59497]

Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (7E10) [NBP2-59497] - Analysis of Leptin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Leptin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)

Applications for Leptin/OB Antibody (7E10) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Alternate Names

LEP, OB

Gene Symbol

LEP

Additional Leptin/OB Products

Product Documents for Leptin/OB Antibody (7E10) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Leptin/OB Antibody (7E10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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