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Lgl1 Antibody (5G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00003996-M01

Novus Biologicals, part of Bio-Techne
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H00003996-M01

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

LLGL1 (NP_004131, 911 a.a. ~ 1010 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GIASCVFTRHGQGFYLISPSEFERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSILLAPQSLDGSPDPAHSMGPDTPEPPEAALSP

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 21785947).

Specificity

LLGL1 - lethal giant larvae homolog 1 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Lgl1 Antibody (5G2)

Western Blot: Lgl1 Antibody (5G2) [H00003996-M01]

Western Blot: Lgl1 Antibody (5G2) [H00003996-M01]

Western Blot: Lgl1 Antibody (5G2) [H00003996-M01] - Western Blot analysis of LLGL1 expression in Hela NE ( Cat # L013V3 ).
ELISA: Lgl1 Antibody (5G2) [H00003996-M01]

ELISA: Lgl1 Antibody (5G2) [H00003996-M01]

ELISA: Lgl1 Antibody (5G2) [H00003996-M01] - Detection limit for recombinant GST tagged LLGL1 is approximately 0.03ng/ml as a capture antibody.

Applications for Lgl1 Antibody (5G2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reviewed Applications

Read 1 review rated 3 using H00003996-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Lgl1

This gene encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

Alternate Names

DLG4lethal giant larvae (Drosophila) homolog 1, HUGL, HUGL1, Hugl-1, Human homolog to the D-lgl gene protein, lethal giant larvae homolog 1 (Drosophila), lethal(2) giant larvae protein homolog 1, LLGL

Entrez Gene IDs

3996 (Human)

Gene Symbol

LLGL1

OMIM

600966 (Human)

Additional Lgl1 Products

Product Documents for Lgl1 Antibody (5G2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lgl1 Antibody (5G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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