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Key Product Details

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG2B Clone # 852229

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Lgr4/GPR48
Met1-Asp951
Accession # Q9BXB1

Specificity

Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Lgr4/GPR48 Antibody (852229) [CoraFluor™ 1]

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Optimal dilutions of this antibody should be experimentally determined.

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Lgr4/GPR48

GPR48 is an Orphan-A GPCR with an unknown ligand. GPR48 expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 4

Alternate Names

GPR48

Gene Symbol

LGR4

Additional Lgr4/GPR48 Products

Product Documents for Lgr4/GPR48 Antibody (852229) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lgr4/GPR48 Antibody (852229) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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