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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 852229

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human Lgr4/GPR48
Met1-Asp951
Accession # Q9BXB1

Specificity

Detects human Lgr4/GPR48 in Lgr4-transfected cell-based ELISA. Stains human Lgr4/GPR48 transfected but not irrelevant transfected cells in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Lgr4/GPR48 Antibody (852229) [PE/Atto594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Lgr4/GPR48

GPR48 is an Orphan-A GPCR with an unknown ligand. GPR48 expression has been documented in human brain, adrenal, heart, kidney, liver, skeletal muscle, ovary, pancreas, placenta, prostate, spleen, stomach, testis, thyroid, and spinal cord.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 4

Alternate Names

GPR48

Gene Symbol

LGR4

Additional Lgr4/GPR48 Products

Product Documents for Lgr4/GPR48 Antibody (852229) [PE/Atto594]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lgr4/GPR48 Antibody (852229) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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