Lgr5/GPR49 Antibody (9J9B8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15445

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15445-100ul
NBP3-15445-20ul
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9J9B8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lgr5/GPR49 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 800-907 of human Lgr5/GPR49 (O75473). VIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Lgr5/GPR49 Antibody (9J9B8)

Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445]

Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445]

Western Blot: Lgr5/GPR49 Antibody (9J9B8) [NBP3-15445] - Western blot analysis of extracts of various cell lines, using Lgr5/GPR49 Rabbit mAb (NBP3-15445) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Lgr5/GPR49 Antibody (9J9B8)

Western Blot: Lgr5/GPR49 Antibody (9J9B8) [Lgr5/GPR49] -

Western Blot: Lgr5/GPR49 Antibody (9J9B8) [Lgr5/GPR49] - Western blot analysis of various lysates using Lgr5/GPR49 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Applications for Lgr5/GPR49 Antibody (9J9B8)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Gene Symbol

LGR5

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody (9J9B8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Lgr5/GPR49 Antibody (9J9B8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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