Lgr5/GPR49 Antibody (CL13567)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24570
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Mouse (91%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL13567
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was designed to target the recombinant protein Lgr5/GPR49, with the precise immunogen sequence being withheld for proprietary reasons.
Reactivity Notes
Rat (87%)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Lgr5/GPR49 Antibody (CL13567)
Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]
Staining of human skeletal muscle shows moderate membranous positivity in myocytes.
Immunohistochemistry-Paraffin: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]
Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Western Blot: Lgr5/GPR49 Antibody (CL13567) [NBP3-24570]
Western blot analysis in human cell line SH-SY5Y.
Applications for Lgr5/GPR49 Antibody (CL13567)
Application
Recommended Usage
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Western Blot
1ug/ml
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Lgr5/GPR49
Long Name
Leucine-rich Repeat Containing G Protein-coupled Receptor 5
Alternate Names
FEX, GPR49, GPR67, HG38
Gene Symbol
LGR5
Additional Lgr5/GPR49 Products
Product Documents for Lgr5/GPR49 Antibody (CL13567)
Product Specific Notices for Lgr5/GPR49 Antibody (CL13567)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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