LIGHT/TNFSF14 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51981

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all LIGHT/TNFSF14 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-HEVNPAAHLTGANS corresponding to internal region according to NP_003798.2, NP_742011.1.

Epitope

C-HEVNPAAHLTGANS

Specificity

This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for LIGHT/TNFSF14 Antibody

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981]

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981]

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981] - Staining of human cerebellum (A) and frontal cortex (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981]

Immunocytochemistry/ Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981]

Immunocytochemistry/Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Applications for LIGHT/TNFSF14 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
WB: Approx. 22 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 22.4 kDa band according to NP_742011.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: LIGHT/TNFSF14

The T5-39 antibody recognizes human LIGHT (CD258) also known as ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, TR2 ligand, and tumor necrosis factor ligand superfamily member 14 (TNFSF14). LIGHT is a type 2 transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. LIGHT can be proteolytically cleaved and two isofoms have been shown to be produced by alternative splicing. The apparent molecular weight of LIGHT us approximately 30 kD. LIGHT expression has been observed in spleen, brain and kidney; expression is upregulated on activated T cells (particularly CD8+ cells). LIGHT stimulates proliferation of T cells and inhibits growth of some tumor cells. This protein has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy. LIGHT is a ligand for HVEM, and LTbetaR and has also been shown to bind DcR3 and modulate function. In addition, LIGHT has been shown to interact with other proteins including TRAF2, TRAF3, and DIABLO. The T5-39 antibody has been shown to be useful for flow cytometry and ELISA.

Long Name

TNF Ligand Superfamily Member 14

Alternate Names

CD258, TNFSF14

Entrez Gene IDs

8740 (Human)

Gene Symbol

TNFSF14

UniProt

NP_003798.2 (Human)

Additional LIGHT/TNFSF14 Products

Product Documents for LIGHT/TNFSF14 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for LIGHT/TNFSF14 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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