LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54645

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all LIM Kinase 1/2 Antibodies »

Product Specifications

Immunogen

A peptide sequence around phosphorylation site of threonine 508 (R-Y-T (p) -V-V) derived from Human LIMK1

Modification

p Thr508

Specificity

This antibody detects endogenous level of LIM Kinase 1/2 only when phosphorylated at threonine 508.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

Western Blot: LIM Kinase 1/2 [p Thr508] Antibody [NBP2-54645] - [p Thr505, p Thr508] Antibody [NBP2-54645] - Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIMK1 [p Thr505, p Thr508] antibody.
Immunohistochemistry-Paraffin: LIM Kinase 1/2 [p Thr508] Antibody - BSA Free [NBP2-54645]

Immunohistochemistry-Paraffin: LIM Kinase 1/2 [p Thr508] Antibody - BSA Free [NBP2-54645]

Immunohistochemistry-Paraffin: LIM Kinase 1/2 [p Thr505, p Thr508] Antibody [NBP2-54645] - Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1 (Phospho-Thr508).Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using LIMK1 (Phospho-Thr508).
LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

Western Blot: LIM Kinase 1/2 [p Thr508] Antibody - BSA Free [NBP2-54645] -

Western Blot: LIM Kinase 1/2 [p Thr508] Antibody - BSA Free [NBP2-54645] - Western blot analysis of lysed extracts from UV-treated C2C12, PMA-treated HeLa and anisomycin-treated 293 cells using LIM Kinase 1/2 (Phospho-Thr508).
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Applications for LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Epitope affinity purified

Formulation

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, and 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: LIM Kinase 1/2

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Alternate Names

EC 2.7.11.1, LIM domain kinase 1, LIM motif-containing protein kinase, LIMKLIMK-1

Gene Symbol

LIMK1

Additional LIM Kinase 1/2 Products

Product Documents for LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIM Kinase 1/2 [p Thr508] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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