LIM Kinase 1 Antibody (1A8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003984-M01

Novus Biologicals, part of Bio-Techne
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H00003984-M01-100ug
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A8

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all LIM Kinase 1 Antibodies »

Product Specifications

Immunogen

LIMK1 (NP_002305, 548 a.a. ~ 647 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

LIMK1 - LIM domain kinase 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for LIM Kinase 1 Antibody (1A8)

Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in NIH/3T3.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - Analysis of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01]

Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in PC-12.
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Applications for LIM Kinase 1 Antibody (1A8)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Sandwich ELISA

1:100-1:2000

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIM Kinase 1

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Long Name

LIM domain kinase 1

Alternate Names

LIMK, LIMK-1, LIMK1

Entrez Gene IDs

3984 (Human)

Gene Symbol

LIMK1

OMIM

601329 (Human)

UniProt

NP_002305 (Human)

Additional LIM Kinase 1 Products

Product Documents for LIM Kinase 1 Antibody (1A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIM Kinase 1 Antibody (1A8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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