LIM Kinase 1 Antibody (2E9)

Novus Biologicals, part of Bio-Techne | Catalog # H00003984-M05

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2E9

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LIM Kinase 1 Antibodies »

Product Specifications

Immunogen

LIMK1 (NP_002305, 548 a.a. ~ 647 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD

Specificity

LIMK1 (2E9)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for LIM Kinase 1 Antibody (2E9)

Western Blot: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

Western Blot: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

Western Blot: LIM Kinase 1 Antibody (2E9) [H00003984-M05] - Analysis of LIMK1 expression in HeLa (Cat # L013V1).
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (2E9) [H00003984-M05] - Analysis of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 3 ug/ml
ELISA: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

ELISA: LIM Kinase 1 Antibody (2E9) [H00003984-M05]

ELISA: LIM Kinase 1 Antibody (2E9) [H00003984-M05] - Detection limit for recombinant GST tagged LIMK1 is approximately 0.3ng/ml as a capture antibody.

Applications for LIM Kinase 1 Antibody (2E9)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for ELISA and IHC-P.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LIM Kinase 1

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Long Name

LIM domain kinase 1

Alternate Names

LIMK, LIMK-1, LIMK1

Entrez Gene IDs

3984 (Human)

Gene Symbol

LIMK1

OMIM

601329 (Human)

UniProt

NP_002305 (Human)

Additional LIM Kinase 1 Products

Product Documents for LIM Kinase 1 Antibody (2E9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIM Kinase 1 Antibody (2E9)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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