LIM Kinase 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87721

Novus Biologicals, part of Bio-Techne
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NBP1-87721
NBP1-87721-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (98%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all LIM Kinase 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHSKLYCGHCYYQTVVTPVIEQILPDSPGSHLPHTVTLVSIPASSHGKRGLSVSIDPPHGPPGCGTEHSHTVRVQGVDPGCMSPDVKNSIHV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22253135)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for LIM Kinase 1 Antibody

Immunocytochemistry/ Immunofluorescence: LIM Kinase 1 Antibody [NBP1-87721]

Immunocytochemistry/ Immunofluorescence: LIM Kinase 1 Antibody [NBP1-87721]

Immunocytochemistry/Immunofluorescence: LIM Kinase 1 Antibody [NBP1-87721] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody [NBP1-87721]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody [NBP1-87721]

Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody [NBP1-87721] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Applications for LIM Kinase 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC, WB reported in scientific literature (PMID: 25237832). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIM Kinase 1

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Long Name

LIM domain kinase 1

Alternate Names

LIMK, LIMK-1, LIMK1

Gene Symbol

LIMK1

Additional LIM Kinase 1 Products

Product Documents for LIM Kinase 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIM Kinase 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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