Key Product Details

Species Reactivity

Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 052

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all LIMPII/SR-B2 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Mouse LIMPII/SR-B2 (Accession#: NP_031670.1; Met1-Thr432).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for LIMPII/SR-B2 Antibody (052)

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Immunocytochemistry/Immunofluorescence: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Staining of mouse SCARB2 in mouse splenocytes. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-mouse SCARB2 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Immunohistochemistry-Paraffin: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Staining of mouse SCARB2 in mouse brain with rabbit monoclonal antibody (1:200).
Flow Cytometry: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Flow Cytometry: LIMPII/SR-B2 Antibody (052) [NBP2-90382]

Flow Cytometry: LIMPII/SR-B2 Antibody (052) [NBP2-90382] - Analysis of Mouse SCARB2 expression on BABL/c splenocytes. The cells were stained with purified anti-Mouse SCARB2, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Applications for LIMPII/SR-B2 Antibody (052)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: LIMPII/SR-B2

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. Although the role of SR-BII is not completely clear, research suggests that it may be a functional HDL receptor. In addition, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Long Name

Lysosomal Integral Membrane Protein II

Alternate Names

CD36L2, LPG85, SCARB2, SR-B2, SR-BII, SRB2

Gene Symbol

SCARB2

Additional LIMPII/SR-B2 Products

Product Documents for LIMPII/SR-B2 Antibody (052)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for LIMPII/SR-B2 Antibody (052)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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