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LINGO-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19359

Novus Biologicals, part of Bio-Techne
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NBP2-19359

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Chicken (92%), Mouse (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LINGO-1. The exact sequence is proprietary.

Localization

Cell membrane; Single-pass type I membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for LINGO-1 Antibody

Immunocytochemistry/ Immunofluorescence: LINGO-1 Antibody [NBP2-19359]

Immunocytochemistry/ Immunofluorescence: LINGO-1 Antibody [NBP2-19359]

Immunocytochemistry/Immunofluorescence: LINGO-1 Antibody [NBP2-19359] - LINGO1 antibody [C1C3] detects LINGO1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LINGO1 stained by LINGO1 antibody [C1C3] diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] diluted at 1:500. Blue: Fluoroshield with DAPI.
LINGO-1 Antibody

Western Blot: LINGO-1 Antibody [NBP2-19359] -

Western Blot: LINGO-1 Antibody [NBP2-19359] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LINGO1 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for LINGO-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: LINGO-1

Functional component of the Nogo receptor signaling complex (RTN4R/NGFR) in RhoA activation responsible for some inhibition of axonal regeneration by myelin-associated factors. Is also an important negative regulator of oligodentrocyte differentiation and axonal myelination.

Long Name

Leucine-rich Repeat And Ig Domain Containing Nogo Receptor-Interacting Protein 1

Alternate Names

LERN1, LINGO1, LRRN6A

Gene Symbol

LINGO1

UniProt

Additional LINGO-1 Products

Product Documents for LINGO-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for LINGO-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.